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atcc 43333  (ATCC)


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    Structured Review

    ATCC atcc 43333
    Atcc 43333, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+43333/Amycolatopsis+orientalis+(Pittenger+and+Brigham)+Lechevalier+et+al/us07655689-430-28-28
    Average 92 stars, based on 45 article reviews
    atcc 43333 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Cloning and expression of a cytochrome P450 hydroxylase gene from Amycolatopsis orientalis: hydroxylation of epothilone B for the production of epothilone F.
    Article Snippet: Degenerate PCR primers were used to amplify cytochrome P450 gene fragments from the high-GC gram-negative bacteria Amycolatopsis orientalis, which catalyzes the hydroxylation of epothilone B to produce epothilone F. The ampliWed fragments were used as hybridization probes to identify and clone two intact cytochrome P450 genes.. The expression of one of the cloned genes in a Streptomyces lividans transformant resulted in the biotransformation of epothilone B to epothilone F. The conversion of epothilone B to epothilone F by the S. lividans transformant was conWrmed by mass spectrometry and nuclear magnetic resonance spectroscopy.



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